 This study investigated the effectiveness of low-level laser therapy, LLLT, for stimulating human gingival fibroblast growth. Fibroblasts were exposed to different energy dosages of LLLT, and their metabolic activity, cell number, and cell migration were measured. The results showed that both 0.5J CM2 and 3J CM2 energy dosages increased metabolic activity, cell number, and cell migration. This suggests that LLLT can be used to promote biostimulation of fibroblasts in vitro. This article was authored by Fernanda G. Basso, Tizer N. Pansoni, Ana Paula S. Turioni, and others.