 The comparison of three next-generation sequencing platforms revealed that the ion-torrent PGM had the best overall performance, followed by the Illumina Micek and then the Pacific Biosciences RS. The ion-torrent PGM showed the most even coverage across all four genomes, while the Pacific Biosciences RS had the lowest coverage on the plasmogeum fulciparum genome due to its high AT content. The ion-torrent PGM also had the highest number of variants detected, while the Pacific Biosciences RS had the lowest. Additionally, the ion-torrent PGM had the fewest context-specific errors, while the Pacific Biosciences RS had the most. Overall, the ion-torrent PGM performed the best out of the three platforms tested, making it the ideal choice for genomic and genetic research.