 This study investigated the effects of two types of mid-task interventions, rest-break and moderate-intensity exercise break, on the dynamic reorganization of functional connectivity, FC, during the execution of psychomotor vigilance test, PVT. Temporal brain networks were estimated before and immediately after the intervention, and towards the end of the task to investigate the immediate and delayed effects respectively during post-break task re-engagement. Behaviorally, similar beneficial effects of exercise and rest-break on performance were observed, manifested by the immediate improvements after both interventions and a long-lasting influence towards the end of tasks. Moreover, temporal brain networks assessment showed significant immediate decreases of flexibility, which was followed by an increase of flexibility towards the end of intervention tasks. Furthermore, the temporal nodal measure revealed the channels with significant differences across tasks were mainly resided in the frontal parietal areas that exhibited interesting frequency-dependent distribution. These observations extend previous fatigue-related intervention and static FC studies, and provide new insight into the dynamic characteristics of FC during post-break task re-engagement. This article was authored by Ling Yun Gao, Jin Yu, Li Zhu, and others. We are article.tv, links in the description below.