 Nanochloropsis selena is a species of algae that can be used for biofuel production. This study examined how different environmental conditions affect the growth rate, fatty acid content, and fatty acid composition of this alga. When grown under various light intensities and temperatures, the alga showed increased fatty acid content and decreased growth rates as the light intensity or temperature increased. Additionally, the fatty acid composition changed depending on the environmental condition, with higher amounts of unsaturated fats when grown under lower light intensities and higher temperatures. This information can help researchers better predict the fatty acid content and yields of algal biofuels. This article was authored by Brayden Crow, Tyler W. Miller, Paul Hook, and others.