 This study used selects a technology to analyze small RNA sequences in normal peripheral blood mononuclear cells, PBMC, and cancer cell lines K562 and HL60. The results showed that K562 cells had low levels of Myrna population and DISA, while highly expressed Myrnas included members of the LET-7 family, MIR-21, 103, 185, 191, and 328. A comparison of Myrna profiles revealed specific differentially expressed molecules, and correlation with mRNA expression profiles identified target genes showing inverse correlation with Myrna levels. The study also predicted novel Myrnas, some of which were validated by real-time RT-PCR and RNA's protection assay. Out of the 904 Myrnas expressed in human eucocytes, 370 are novel. The study suggests that post-transcriptional processes may play a significant role in regulating levels of Myrnas in tumor cells and provides a customized automated computation pipeline for Myrna profiling and identification of novel Myrnas. This article was authored by Kuma Lullet, Gupta Roshi, Shama Pratiba, and others. We are article.tv, links in the description below.