 Microfluidic systems are becoming increasingly popular due to their ability to perform complex tasks in small spaces. To ensure successful operation, these systems require accurate temperature control. Over the past decade, several techniques have been developed to achieve this goal, including external heaters, joule heating, microwave heating, and laser heating. These methods can be used to create temperature gradients, uniform temperatures, or even combinations of the two. Additionally, certain techniques have been developed specifically for applications like digital microfluidics, polymerase chain reaction, and temperature gradient focusing. Regardless of the application, all of these techniques require precise temperature control, which can be achieved through careful design and implementation. This article was authored by Marie-Caroline Gillian, Florent Melodgie, Axel Huair, and others.