 Sulphur cycling is a primary biogeochemical process in nature, and prokaryotic oxidation drives a major part of it. Reduced inorganic sulfur compounds, such as elemental sulfur and tetrothionate, among others, can be used as sole energy sources by acetylphilic bacteria like acetytoacillus teoxidens. In biosigma, we have developed a metabolic model based on genomic, as well as experimental information that will help us to understand how this energy process takes place and what are the key metabolic steps involved. This model can be used to unravel in part the microbial activity that takes place in industrial mining operations, where biological sulfur oxidation supports the recovery of Balovoo metals.