 DNA barcoding is a powerful tool for identifying species and understanding biodiversity. It has been applied to a variety of taxa, including vertebrates, but its effectiveness depends on the choice of DNA sequence. This paper proposes a novel method for evaluating the quality of DNA sequences used for barcoding, based on in silico PCR tests. These tests were performed on various DNA regions proposed for barcoding, and compared to in vitro PCR tests. The results show that in silico PCR can be used to optimize the performance of a study, by allowing the selection of the best barcode depending on the study's goals. This article was authored by Scheherzad Waseem, Riyaz Tiiba, Zandal Stephanie, and others.