 This paper provides a comprehensive guide for systematic review and meta-analysis of observational studies. The authors first discuss the importance of observational studies in evidence synthesis and then outline seven steps to systematically integrate evidence from these studies. These steps include defining the scope of the review, identifying relevant studies, extracting data, assessing risk of bias, summarizing results, exploring heterogeneity, and interpreting results. The authors also discuss potential pitfalls and caveats when conducting such reviews. This paper provides a useful resource for those interested in conducting systematic reviews and meta-analyses of observational studies. This article was authored by Maria Glyzik, Maria Glyzik, Peter Francis Ragwinden and others.