 The study aimed to improve the stability and immunogenicity of a BCG-vectored HIV vaccine by using integrative expression vectors and an antibiotic-free plasmid selection system based on double oxotrophic complementation, resulting in a more stable and immunogenic vaccine that can be used to prime protective responses against other major pediatric pathogens. This article was authored by Akash Mahant, Narciss Sorbi, Yoshiki Ito, and others.