 Zinc oxide nanoparticles, ZNONPs, have gained significant attention in the biomedical field due to their unique properties, including biocompatibility, low cost, and low toxicity. They have been widely used in various applications, such as anti-cancer, antibacterial, and anti-diabetic treatments. Additionally, they can be used for imaging purposes due to their luminescence properties. Recently, there has been much progress made in the synthesis and application of ZNONPs in the biomedical field. This review provides an overview of the current status of this rapidly developing area of research. This article was authored by Gene Wang, Jiang, Jiang Pai, and GACAI.