3D Reconstruction of Frozen Plant





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Uploaded on Jun 3, 2010

For the anaglyph version of this video go to: http://www.youtube.com/watch?v=d0gHEl...

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This is an example of using color keying to digitally clear plant tissue and allow visualization of internal structures in 3D. For another example see Arabidopisis flower in 3d at: http://www.youtube.com/watch?v=zlw-50... . The technique is ideally suited for in situ hybridization analysis to detect mRNA in an anatomical context

This is not a cartoon. This 3D volume was reconstructed from JPEG photographs of individual slices of plant tissue stained with Safranin and Fast Green. Photos were taken with a consumer-grade camera (Sony DSC707) mounted on a Nikon Eclipse 50i microscope using a 4X plan apo objective.

JPEG images were imported into Adobe After Effects where they were aligned and the 3D volume was constructed. The full HD version is ½ the full resolution available. When the technology catches up, we will re-post at higher than 1080.

Distortion-free sections were prepared by Research Associate Tan Tuong. Graphic consulting was provided by Randy Gelman, BeltlineMedia, http://www.beltlinemedia.com

For more information see Livingston et al 2010. 3D volumes constructed from pixel-based images by digitally clearing plant and animal tissue. Journal of Microscopy. PDF was on my website at:: http://www.ars.usda.gov/Main/docs.htm..., but video embedded in PDF did not work. Will post soon.

Contact: David Livingston, dpl@unity.ncsu.edu 919-515-4324

Tags: anaglyph" '3D" "red-green glasses" histology "in situ hybridization" "Nikon Eclipse 50i" "Adobe After Effects" "digital clearing" microscope "JPEG images" "serial sections" "frozen plant" "oat crown"


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