 The study aimed to develop a nanomodule formulation that would enhance the rheological behavior and physical chemical properties of mupiricin and muP to improve its skin permeability, but found that both nanomodules exhibited lower skin permeability compared to the marketed control. The local accumulation efficiency of mup from nanomodule was significantly higher than that of the control after eight hours and micro CT scan imaging allowed visualization of these findings. While there were no significant differences in antibacterial activities between the nanomodules and the control, the nanomodules demonstrated superiority over the control due to their lower content of mup. The potential use of the nanomodule for targeting skin lesions where high skin deposition and low permeability are required is supported by these findings. This article was authored by Badged El-Hasso, Muhammad Usman Gori, and Barbara R. Conway.