 This study investigated the effects of low-intensity pulse ultrasound, lipos, combined with lipid microbubbles, LM, on polylactic glycolic acid copolymer, PLGA, tricalcium phosphate, TCP, 3D-printed scaffolds. The results showed that lipos combined with LM could increase the proliferation of bone marrow mesenchymal stem cells, BMSCs, as well as the expression of type 1 collagen and alkaline phosphatase activity. Furthermore, it also promoted the formation of mineralized matrix and osteoblastic differentiation. This suggests that lipos combined with LM may be a promising approach for bone regeneration in tissue engineering. This article was offered by Lin Jin, Jelly Sean, Yan Honghao, and others.