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Fast Sequential Imaging with the Tiberius® fs Laser and Bergamo® II Multiphoton Microcope

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Uploaded on Nov 20, 2017

https://www.thorlabs.com/newgrouppage...
With an industry-leading tuning speed of up to 200 nm in less than 50 ms, the Tiberius is ideal for fast sequential imaging in multiphoton microscopy applications. The ability to rapidly switch the excitation wavelength allows users to obtain high-contrast, multi-fluorescence images.

This video shows the sequential excitation of a 25 µm thick adult rat brain saggital section. The red channel corresponds to fluorescence from chick anti-neurofilament excited at 835 nm, while the green channel corresponds to fluorescence from mouse anti-GFAP excited at 750 nm. At full speed, both channels are collected at an imaging rate of 7 fps; this video is shown at 1/16 of the full speed.

This immunofluorescence sample was prepared by Lynne Holtzclaw of the NICHD Microscopy and Imaging Core Facility, a part of the National Institutes of Health (NIH) in Bethesda, MD.

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