 Hi everyone. Welcome to basic science series. I'm your host Lokinder Kumar and today We are going to discuss how to write materials and method section of the manuscript So today I'm going to discuss the specific part that is materials and method section Although I made videos on how to write title of our manuscript how to write abstract introduction of the manuscript I have explained all those components by giving Examples of my own research article. Before we discuss the topic I want to request that if you are new to the channel Please do subscribe to the channel and if you like the video then press the like button You can also support me on patreon.com. I'll post the link of my patreon website in the description box So whenever you perform a specific experiment There are three important component of that experiment Component number one is the instrument component number two is the chemical or the supplies Component number three is the protocol So when you write the materials and method section of your manuscript you need to focus on these three components Very precisely So we're going to discuss all three components one by one. So let's discuss component number one, which is instrument An instrument is the device that is used during the experiment to collect the data as well as Sometime to analyze the data if you are taking an example of micro dilution method using a micro titled plate So you will need a Eliza a great reader or Generally, you will you will require a plate reader So what plate reader will will do it is going to take optical density measurements of your bacterial culture And it's going to tell you that how your bacteria is Growing under the concentration of the antimicrobial agent. So if you're using a plate reader So in this case, you might be using a specific plate reader from a specific company So in materials and method section, you have to mention the model number and The name of the company that is going to help all the researchers that are trying to reproduce your experiment So it is really important that you mention the details of the instrument So that point Mentioned the importance of the instrument and the details of the instrument second important point is the supplies or The chemicals or the reagents that you're going to use So whenever you use a particular reagent for example in this case, you're going to use Antibiotics or antimicrobial agents that you want to test Right, so you need to mention the made and you need to mention the Item number. So when you see that particular product, you're going to see that there will be The catalogue number of that particular chemical mentioned on that particular while so you need to specifically give the details of those chemical compounds that you are testing Protocol is the third important part of the materials and method section In protocol, you need to mention. What are the different different steps that you have followed? For example, how much? the incubation time for the bacterial cultures and How long you have incubated? What is the temperature? So these are the important critical parameters that you need to mention in that protocol the idea of Materials and method is so that someone else who is trying to follow the protocol Should reproduce your protocol. So the idea is to provide all the necessary details So basically these three things are the most important points in Materials and method section of the manuscript you need to mention the details of the instrument What is the model number? What is the company of the instrument? Second you need to mention the details of the supplies for example The materials that you have used in the experiment. It can be a chemical. It can be a solvent It can be a compound that you have used in the experiment Third important part is the protocol that how you have followed your experiment and how you have achieved those results so When you write all those things in a documented format That becomes the material and method section of your manuscript All right, so that was the basic information that you can use to write the materials and method section of your manuscript and Always pay attention to the details because details are really important while you're writing materials and method section I would raise a very important point here is you can write a lengthy material method section and put that material in method section in the supplementary section of the manuscript So if someone wants to reproduce your experiment then he or she can go to the supplementary section and then download the file Because most of the time supplementary section of the manuscript allows you to add like huge data files and it doesn't have a limit but in case of the main Manuscript text you have a specific word limit. So I would suggest you use the supplementary section of the manuscript and provide all the details for the materials and method section of your manuscript So, please stay tuned To the channel and if you're new to the channel, I Request you to subscribe to the channel that will encourage me to make more videos. Thank you and take care