 Nanotechnology has emerged strongly in many fields of science, with atoms and molecules working differently at a tiny scale. This presents a variety of exciting and promising applications, such as pharmaceutical nanocarriers. These nanoparticles, nanospheres, nanoemulsions, nanoliposomes, and nanoniosomes can be designed for controlled delivery of drugs or other substances. To ensure successful delivery, it is important to understand how these nanocarriers behave in vitro and in vivo. Characterizing them involves measuring their size, morphology, and surface charge, as well as determining their colloidal stability, zeta potential, and binding internalization to cells. Additionally, bio-distribution studies can be conducted to assess the intracellular uptake and subcellular localization of the nanocarrier. This article was authored by Oculus K. Jain and Shuresh Peraja.