 Miniaturization of diagnostic tools for biomolecular detection has become increasingly important due to the need for improved sensitivity, selectivity, and high throughput. One such tool is nucleic acid amplification, which can be used to detect a limited amount of target molecules. Microfluidic devices are being developed to reduce the required sample volume and analysis time, as well as offer automated and integrated processes. Most of these devices utilize polymerase chain reaction, PCR, but isothermal amplification methods have been developed that do not require thermal cycling. These methods are more suitable for miniaturized analysis systems as they eliminate the need for complex temperature control. This article was authored by Giuseppe Spotto and Laura Maria Zinoli.