The handling and care of cryopreserved hepatocytes is crucial to achieving consistent cell yield and viability from any lot. This video guide provides step-by-step instructions on the proper way to thaw, plate, and incubate cryopreserved hepatocytes for optimal results.
Reference:
Thawing and Plating Protocol - http://triangleresearchlabs.net/wp-co...
Cell Counting Protocol : Trypan Blue Exclusion Method - http://triangleresearchlabs.net/wp-co...
About Triangle Research Labs:
Since its launch in 2011, Triangle Research Labs, or TRL, has supported scientists by providing high quality hepatocytes for multiple research applications. Based in Research Triangle Park, NC, TRL produces fresh and cryopreserved hepatocytes and hepatic non-parenchymal cells from a variety of species in-house. Additionally, TRL distributes cryopreserved NoSpin HepaRG™ cells and media for Biopredic International, and QuasiVivo® Cell Culture Flow Systems for Kirkstall, Ltd in the US and Canada. TRL utilizes an in-depth understanding of research needs to provide efficient solutions. Visit http://triangleresearchlabs.net/ to learn more.