 Abstract hexagonal boron nitride, HBN, is a unique two-dimensional, 2D, material that holds solid state spins and has great potential for use in quantum information applications such as quantum networks. In these applications, both optical and spin properties are important, however, they have not yet been discovered simultaneously for HBN spins. We developed an effective method for arraying and isolating single defects of HBN and found a new spin defect with a high probability of 85%. This single defect exhibited excellent optical properties and was able to be optically controlled through rubby oscillations and Haneco experiments at room temperature. Calculations suggest that carbon and oxygen dopants could be the source of the single spin defects, providing a pathway for further exploration into optically controlling spins. This article was offered by Nijie Kwa, Song Lee, Wei Yi Liu, and others. We are article.tv, links in the description below.