Loading...

Biomolecular Interaction Analysis with the ProteOn™ XPR36 System

1,198 views

Loading...

Loading...

Transcript

The interactive transcript could not be loaded.

Loading...

Loading...

Rating is available when the video has been rented.
This feature is not available right now. Please try again later.
Published on Dec 10, 2012

For more info, visit http://www.bio-rad.com/yt/7proteonxpr36.
Surface plasmon resonance (SPR) is employed as an excellent technology for measuring biomolecular interactions in real-time in a label-free manner. Using this method, one of the interacting partners (ligand) is immobilized to the sensor surface, while the other (analyte) is free in solution and passed over the surface. The kinetic parameters of the interactions are further calculated from association and dissociation sensorgrams. This lecture introduces the ProteOn XPR36 system, an SPR optical biosensor featuring a 6 x 6 interaction array for the simultaneous analysis of up to six ligands with up to six analytes which enables the analysis of up to 36 different protein interactions in one run on a single chip. A protein-protein interaction, of adenylate cyclase toxin (CyaA) with integrin receptor CD11b/CD18, a lipid-protein interaction, of phosphatidylinositol 4,5-bisphosphate (PIP2) with membrane channel TrpV1, and a DNA-protein interaction, of transcription factor FoxO4 with target DNA, are presented to demonstrate the throughput, flexibility, and versatility of this instrument.

Presenter:
Dr. Ladislav Bumba
Institute of Microbiology AVCR
Prague, Czech Republic

http://www.bio-rad.com/evportal/desti...

We Are Bio-Rad.

Our mission: To provide useful, high-quality products and services that advance scientific discovery and improve healthcare. At Bio-Rad, we are united behind this effort. These two objectives are the driving force behind every decision we make, from developing innovative ideas to building global solutions that help solve our customers' greatest challenges.

Connect with Bio-Rad Online:
Website: http://www.bio-rad.com/
LinkedIn: https://www.linkedin.com/company/1613...
Facebook: https://www.facebook.com/biorad/
Twitter: https://twitter.com/BioRadLifeSci
Instagram: @BioRadLabs
Snapchat: @BioRadLabs

Loading...

When autoplay is enabled, a suggested video will automatically play next.

Up next


to add this to Watch Later

Add to

Loading playlists...