 The study examines the technical variability in RNA-SEC experiments using solexa, Illumina sequencing technology. The authors found that large disagreements between technical replicates occur when coverage is low, and exon detection is highly variable at this level of coverage. They also noted that estimates of gene expression are more likely to disagree when coverage is low. The study suggests that technical variability results in inconsistent detection of exons and can substantially disagree with the estimate of relative abundance even when coverage levels are high. The authors provide practical recommendations for dealing with technical variability without significant cost increases. This article was authored by Obergenel, Amin Victor, Morse Allison M, and others. We are article.tv, links in the description below.