 Aconidum carmachileae, commonly known as monkshood or aconite, is a plant native to Asia and Europe. It has been used for centuries in traditional Chinese medicine to treat various illnesses, but recent studies have shown that it can be highly toxic when taken in large amounts. This study investigates the effects of aconidum carmachileae on zebrafish larvae, focusing on the neurotoxic mechanism of this plant. The results show that high doses of aconidum carmachileae induce excessive reactive oxygen species, ROS, production, which leads to neuronal damage and cell death. Furthermore, the study suggests that mitochondrometeated apoptotic signaling pathways may play a role in the neurotoxic mechanism of aconidum carmachileae. This article was authored by Majun Pan, Shinsong, Yeridongmiao and others.