 This research has developed a novel digital microfluidic, DMF, system, which allows for on-demand droplet control. A phase modulator was used to cover the entire depth of the microfluidic channel in one shot, allowing for 95% of particles in the droplet to be captured in just three shots. Additionally, shaking processes were employed to ensure that all particles were captured. Using this system, individual yeast cells could be observed and cultured in an array of chips. Single cell sorting, cultivation, and analysis were also possible, demonstrating the heterogeneous nature of yeast cells and proving the effectiveness of the DMF system. This article was authored by Chushu Chen, Qi Meng, Yu Jilu, and others.