 SDIN Toolbox 2.0 is a software package that automates complicated spatial analyses using an intuitive graphical user interface, allowing researchers to use the most current ARC GIS and Maxent software, reduce development time for common solutions, and includes pre and post-processing tools for species distribution modeling, biodiversity quantification, and other macroecology, landscape genetics, and evolutionary studies. This article was authored by Jason L. Brown, Joseph R. Bennett, and Conor M. French.