 The UIO-66NH2MOF-PAMMEM modified GCE showed excellent electro-catalytic performance towards tramadol detection. It was able to detect tramadol in a wide concentration range from 0.5M to 500M with a narrow limit of detection of 0.2M. Additionally, the sensor had good stability, repeatability, and reproducibility. Furthermore, the UIO-66NH2MOF-PAMMEM modified GCE displayed satisfactory practical ability in pharmaceutical formulations, tramadol tablets, and acetaminophen tablets. This article was authored by Fariba Garkhani-Nijad, Hadid by Tallahai, and Iran Shikshouay.