 The study aimed to explore the generalizability of potential prognostic biomarker candidates for hepatocellular carcinoma, HCC, in independent datasets. The authors cross-validated markers of overall survival, OS, and vascular invasion in three datasets, including ionasec, affymetrix arrays, and Illumina gene chips. They found that only a few genes remained significant after multiple testing correction, with BIRC5, CDC20, and PLK1 being the best performing genes in Asian patients. However, none of these genes were significant in the Caucasian cohort. In a multivariate analysis, stage, expression of CNPH, and CDK4 were found to be significant predictors of OS. KIF18A was the only gene that predicted vascular invasion in both the affymetrix and Illumina cohorts. Overall, the study suggests that about half of biomarker candidates failed to retain prognostic value and none were better than stage at predicting OS. This article was authored by Othelia Menihart, Adam Nagy, and Baalish GYFFY. We are article.tv, links in the description below.