 The biosynthesis method is thought to be a clean, non-toxic and environmentally friendly way to produce silver nanoparticles. It involves using certain types of microorganisms to create these particles from a solution containing silver ions. These microorganisms can then be used to control the size of the particles, with smaller particles being more desirable due to their increased surface area and high antimicrobial activity. In this study, the fungus fusarium oxysporum was chosen because it produces the smallest particles when treated with specific concentrations of silver nitrate and incubation temperatures. This treatment also results in the highest antimicrobial activity against both ischoricia coli and staphylococcus aureus. Additionally, the cytotoxicity of the silver nanoparticles was measured and found to have an IC50 value of 121.23 gCm3. This article was authored by Sheriff Musa Hussaini, Tohera Esala and Hende Antia. We are article.tv, links in the description below.