 This study examined the effects of Propofol on the brain's consciousness states using a combination of EG, blood drug concentration, and behavioral response indicators. It found that the EG microstate sequences differed significantly between two different levels of sedation, suggesting that hidden layers may exist in the brain during these transitions. This suggests that monitoring the consciousness states of patients and ensuring the appropriate depth of anesthesia, DOA, is critical for the safe implementation of surgery. This article was authored by Jian Liu, Licheng Xixi, Weiwei Su, and others.