 This study presents a novel bacteriophage-based nanobiosensor for the rapid and accurate detection of E. coli-01-57-H7 in food samples. The electrochemical impedance spectroscopy was used to optimize the sensor's performance by incorporating AUNPs, MWCNTs, and W03 nanomaterials into the screen-printed electrode. This allowed for high sensitivity towards the targeting bacterial host cells while maintaining high specificity against other bacterial strains. The sensor was then used to detect E. coli-01-57-H7 in food samples with a limit of detection of 3C-FU-ML and a recovery rate of 90% to 108%. As such, this bacteriophage-based nanobiosensor can be used as a disposable label-free impedimetric biosensor for the quick and real time monitoring of food quality. This article was authored by Nader Abdelamid, Fatma Abdel-Romin, Aiman El-Shibani, and others.