 This study investigated the effects of drought on micro-RNA, Myrna, expression in the legume species Medicago truncatula. Using high throughput sequencing, 22 Myrnas were found to be up-regulated and 10 Myrnas were found to be down-regulated under drought conditions. Additionally, 238-putative novel Myrnas were identified, as well as 14 previously unknown Myrnas belonging to four different Myrna families. Furthermore, the targets of these Myrnas were found to be involved in various biological processes such as development, transcription, protein degradation, detoxification, and nutrient status. This study provides valuable insight into the mechanisms underlying drought tolerance in this legume species. This article was authored by Jiang Minghui, Chen Lei, Wang Qianzuo, and others.