 Low-temperature lamp is a novel version of loop-mediated isothermal amplification lamp. It operates at 37 degrees Celsius instead of the standard 65 degrees Celsius, making it suitable for use in field settings where reagents may be limited. This technique requires fewer DNTPs than conventional lamp and uses shorter DNA probes and different concentrations of magnesium sulfate and DNTPs. When optimized, the amplification efficiency of low-temperature lamp is comparable to that of conventional lamp. Additionally, the ligation step can be performed at 37 degrees Celsius without affecting the temperature, allowing for detection of Mir-21, a cancer-promoting oncogenic microRNA. This technique could have wide applications in areas such as point-of-care testing and facility-limited settings. This article was authored by Daehan Nam, Siye Chin Kim, Yongho Kim and others.