 Nanoparticle technology has been used to develop novel antimicrobial agents that target multi-drug resistant organisms, MDROs, such as enterococcus fissium, Staphylococcus aureus, Clebsiela pneumonii, Acinetobacter bomanii, Pseudomonas aeroginosa, and enterobacter species. These particles have demonstrated in vitro antimicrobial activity against these pathogens, and when combined with appropriate antibiotics, they have shown synergistic effects. Additionally, nanoparticles have been found to be able to penetrate the cell membranes of pathogenic microorganisms, disrupting their essential molecular pathways and leading to death. This review discusses the various types of nanoparticles that have been studied, their pharmacokinetic and pharmacodynamics properties, and how they interact with MDROs. This article was authored by Nanyao Li, Nanyao Li, Wenqian Ko, and others.