 The genome-scale metabolic model, GSMM, of Vibrio-Natrigans, ILC 858, was created using an automated draft assembly and extensive manual curation. It was then validated against empirical data from mass spectrometry-based proteomics, and compared to the metabolic model of Escherichia coli. The model revealed several unique features of V. Natrigans, including its ability to use sodium as an energy source and its adaptation to high salinity environments. Additionally, the model was used to create a resource balance analysis which showed how carbon resources are allocated within the cell. This model provides valuable insight into the metabolic capabilities of V. Natrigans and can be used to inform future metabolic engineering efforts. This article was authored by Lucas Coppens, Tanya Cherhard, Dagmar H. Leary, and others.