 Grilus bimaculatus is an emerging model organism used in various fields of biology, including behavior, neurology, physiology, and genetics. Recent advances in reverse genetics have enabled researchers to manipulate gene expression and gain insight into the functional genomics of this species. In particular, the use of CRISPR slash Cas9 has allowed for the efficient knockdown of two key proteins involved in insect melanin production and catecholamine biosynthesis, tyrosine hydroxylase, TH, and yellow, yellow-y. Knockdown of these proteins results in defective pigmentation and defective wing and leg development, respectively. Furthermore, the inheritance of the mutant alleles was observed in the F0 generation, indicating that TH and yellow-y are essential for normal development and survival of G bimaculatus. This article was authored by Yun Bai, Yuan, He, Chu Xie Shen, and others.