 Abstract, the gaseous molecule nitric oxide, NO, plays a crucial role in signaling pathways involved in numerous physiological processes such as vasodilation, neurotransmission, inflammation, apoptosis, and tumor growth. Its short half-life and concentration dependence restrict its function to target sites. Exogenous NO can promote cellular functions and treat various pathological conditions. NO releasing platforms or donors have a advanced in recent years to deliver NO in a controlled and sustained manner to target tissues or organs. This review article discusses the generation of NO via enzymatic functions of NO synthase and from NO donors and the multiple biological and pathological processes that NO modulates. The methods for incorporating NO donors into diverse biomaterials are summarized and these NO releasing platforms are highlighted in terms of advancing treatment strategies for various medical problems. This article was authored by Syed Montazir and Rabi, Navadashree Sharma, Anik Karan, and others. We are article.tv, links in the description below.