 The study demonstrates the effectiveness of combining citizen science and environmental DNA, EDNA, metabarcoding methods in describing groundwater amphipod distribution, abundance, and diversity. While citizen science is effective in detecting amphipods, EDNA sampling allows for assessment of the entire groundwater community, including microorganisms. The study found distinct correlations between amphipod diversity and overall biodiversity of groundwater organisms detected by EDNA at each site, suggesting that these approaches can provide valuable insights into subterranean biodiversity. This article was authored by Marjorie Couton, Angela Studer, Samuel Hurlman, and others.