 One trick for pipetting the accurate amount is when you put, I mean especially, this is especially important for smaller volumes such as anything from one microliter to ten microliters, but it's also, you can use this trick for all of the micro-pipettes as well. So for Albi micropipetting five microliters of liquid. In the proper way to do it, so it's very accurate that you do have exactly five microliters. You go to that full stop like I, that first stop that I explained earlier, go right to the below to your liquid. Don't go all the way down or go all the way, or you need to be in the liquid, of course, and don't try to go to the side, but be straight right in the middle. And then you, you were at that first stop and you pull the liquid up. So now you have five microliters. And when, and this is such a small amount that the best way to try to make sure that you're actually getting everything out in your Eppendorf tube, or any other tube really, I would go to the side, go to that first stop, and then to that second stop, and there you go, you have your five microliters in your Eppendorf tube.