 single-cell ionasequencing, SC-ionasec, provides valuable insights into human islet cell types and their corresponding stable gene expression profiles. However, this approach requires cell dissociation which complicates its utility in vivo. On the other hand, single-nucleus ionasequencing, SN-ionasec, has compatibility with frozen samples, elimination of dissociation induced transcriptional stress responses, and affords enhanced information from entronic sequences that can be leveraged to identify pre-m-ionase transcripts. We obtained nuclear preparations from fresh human islet cells and generated SN-ionasec datasets. We compared these datasets to SC-ionasec output obtained from human islet cells from the same donor. We employed SN-ionasec to obtain the transcriptomic profile of human islets engrafted in immunodeficient mice. In both analyses, we included the entronic reads in the SN-ionasec data with the GRCH38-2020, a library. First, SN-ionasec analysis shows that the top for differentially and selectively expressed genes in human islet endocrine. This article was authored by Randy B. Kang, Yang Sway Lee, Carolina Rosslet, and others. We are article.tv, links in the description below.