 The optimal enzymatic cocktail for hydrolyzing AFEX-pre-treated corn stover consists of a combination of two xylonases, LX3 and LX4, and three cellulases, CBHI, CBH2 and EGI, at a mass ratio of 25 to 75. Additionally, the addition of 0.6 milligrams per G-glucon of xylosidase, LX, and 0.6 milligrams per G-glucon of arabinofuranosidase, LARB, increases the monomeric xylos yield by 20%, while the addition of 0.8 milligrams per G-glucon of glucuronidase, LGL, further increases the xylos yield by another 20%. Furthermore, this cocktail has been shown to reduce the total enzyme loading required for equivalent yields when compared to Accelerace 1000, a commercially available enzyme cocktail. This article was authored by Hermansen Spencer, Ushiro, Chanda Watshushi PS, and others.