 Barcode Fusion Genetics yeast 2 hybrid, BFG-Y2H, is a new method for screening large numbers of protein pairs simultaneously. It combines the advantages of yeast 2 hybrid technology with the power of next-generation sequencing to increase the efficiency of protein matrix screening. This method has been successfully used to screen up to 2.5 million protein pairs, demonstrating its potential as a powerful tool for high throughput protein interaction mapping. This article was authored by Nozomuyashi, Evangelia Petzalaki, Joseph C. Mella, and others.