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EasyBlot: the easiest way to get the best result.

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Published on Feb 27, 2013

EasyBlot, finally a solution to masking by denatured IgG!
http://www.genetex.com/WebPage/Public...
Immunoprecipitation (IP) and co-Immunoprecipitation (co-IP) assays often necessitate the use of the same, or same-host, antibodies for both the IP and western blot steps of these procedures. In these situations the target protein signal can be partially or completely masked on the western blot by secondary antibody detection of the denatured heavy and light chains of the IP antibody. GeneTex's collection of EasyBlot secondary antibodies eliminates this problem by binding only to the non-denatured primary antibody used for the western blot while ignoring the denatured IgG chains of the IP antibody. In addition, GeneTex's EasyBlocker reagent eliminates background signal caused by free Protein A or Protein G. Together, these reagents will help you to get the cleanest results on your IP/co-IP experiments.

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