 Abstract towels are unique in their fiber structure and usage compared to other textiles. They can be used for up to six months before they begin to show signs of odor and dullness. During this period, biofilm formation occurs on the towels, which is composed of polysaccharides, proteins, nucleic acids, and viable counts. These changes are associated with the towels dullness and odor intensity. Additionally, certain types of bacteria have been found to be more prevalent on these towels than on human skin or clothing. This suggests that specific bacterial species may be adapted to the towel fiber environment and contribute to the development of biofilms. This article was authored by Haroro Kato, Nejiza Okino, Hiroki Kijitori, and others.