 Coherence is a crucial aspect of various processes, including energy transfer in supermolecular nanostructures and quantum optical applications. Coherent control schemes exploit this property by manipulating outcomes of dynamical processes but measuring single quantum systems at elevated temperatures in solid matrices requires a reliable approach to retrieve relevant parameters governing ultrafast coherent dynamics. This study presents measurements of the ultrafast coherent decay of two photon-accessible excited states in single organic molecules embedded in a disordered environment at room temperature and uses a quantum dynamics identification procedure to develop a minimum three-level model for accurate description of the data. The results demonstrate sensitivity of the ultrafast excited state dynamics to local nano-environment from molecule to molecule, providing a robust approach to measure and analyze ultrafast quantum dynamics in complex nanosystems. This article was authored by Kevin Wilmer, Chuankang Xu, Ollrich Sturff, and others. We are article.tv, links in the description below.