 Centrioles are essential for the formation of cilia and flagella, as well as for the organization of microtubules within the cell. These microtubules are important for cell shape, polarity, motility, and division. Super-resolution microscopy has been used to analyze the spatial relationships between centrioles and other components of the centrosome, such as the paracentriolar matrix, PCM. During mitosis, the PCM forms extended networks with interspersed tubulin, while during interphase, many proteins are arranged at specific distances from the walls of centrioles, resulting in ring-staining, often with discernible density masses. The C-terminal region of CEP-152 is closer to centrioles than the N-terminal region, demonstrating the power of super-resolution microscopy to study protein disposition. Appendage proteins show rings with multiple density masses, and the number of these masses is greatly reduced during mitosis. At the proximal end of centrioles, SARS-6 forms a dot at the site of daughter centriole assembly, consistent with its role in cartwheel formation. PLK4 This article was authored by Katarina F. Sonnen, Lothar Skamella, Heinrich Leonhardt, and others. We are article.tv, links in the description below.