 Biotech-produced carotenoids play an important role in scientific research due to their antioxidant properties and ability to act as natural pigments. Research has focused on developing more sustainable processes for extracting these compounds from microbes, including using organic solvents, ultrasonics, microwave energy, ionic liquids, and eutectic solvents. Additionally, green recovery methods for producing high-value applications like novel foods and pharmaceuticals are being explored. Finally, methods for identifying and quantifying carotenoids are discussed to provide a roadmap for successful extractions. This article was authored by Harris Papapostolo, Vasiliki Kakramanadu, Maria Alexandri, and others.