 This study examined the frequency of markers of transfusion transmitted infections among blood donors in a blood bank at a tertiary care hospital. A total of 29,732 donors were screened for hepatitis B, hepatitis C, HIV, 1 and 2, syphilis and malaria. Out of these, 2,587 donors were found to be positive for one or more of these infections. The overall prevalence of infectious diseases was 8.6%. The highest prevalence was seen in anti-HCV, 3%, followed by HBSAG, 2.9%, syphilis, 2%, HIV, 0.5%, and malaria, 0.02%. This study highlights the importance of screening blood donors for transfusion transmitted infections and suggests the further research should be done to determine the risk factors associated with these infections. This article was authored by Hirakadir, Nadia Nasir, Shaheen Kauser, and others.