 The research evaluated the adjuvantability of PCPI, a natural plant polysaccharide derived from poria cocos, in enhancing the immunogenicity and protection of an anthrax vaccine using the anthrax protective antigen, PA, as a model. PCPI significantly increased anti-PA antibodies, toxin-neutralizing antibodies, anti-PA antibody affinity, IgG1 and IgG2 are levels, and the frequency of PA-specific memory B cells, proliferation of PA-specific splenocytes, Illinois, for secretion, and DC activation in vitro. The combination of PCPI and CPG-enhanced anti-PA antibodies and neutralizing antibodies, particularly PA-specific IgG2a, shifted the TH2, biased to a TH1, TH2-balanced response, and improved the survival rate of immunized mice following challenge with the anthrax lethal toxin. These findings suggest that PCPI may be a promising vaccine adjuvant that warrants further study. This article was authored by Kuan Liu, Yin Yin, Zhang Zhong, and others. We are article.tv, links in the description below.