 This research focuses on modelling the physiological response of human melanoma and forcing fibroblast cells to multi-layer zinc oxide nanomaterials under UVA exposure, which results in significant cell necrosis due to a loss in mitochondrial membrane potential and intense reactive oxygen species fluorescence. The findings of this study provide valuable insights for biomedical and clinical applications. This article was authored by Muhammad Fakha Yalam, M.Waseem Akram, Seema Bikbal, and others.