 WHO urged for an extension of screening and testing due to the widespread presence of the coronavirus SARS-CoV-2. As such, a rapid and reliable diagnostic method is required. We developed a reverse transcription loop-mediated isothermal amplification, RT-Lamp, assay to detect SARS-CoV-2 in 30 minutes. For sets of primers were designed, targeting the viral RNA of SARS-CoV-2 in the regions of ORF1AB, S-gene, and N-gene. A colorometric change indicated the presence of viral RNA, allowing for easy visualization of results. The assay was tested on 16 clinical samples, with eight positive and eight negative results. The test results were consistent with those obtained using real-time quantitative polymerase chain reaction, RT-QPCR. Additionally, we demonstrated that the RT-Lamp assay could be performed without RNA extraction, making it suitable for use in remote locations. This rapid, reliable, and cost-effective RT-Lamp assay provides a promising solution for. This article was authored by Wei Yi Huang, Boon Lim, Kieh Chen Hsu, and others. We are article.tv, links in the description below.